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plasmid vectors plenticas9 t2a gfp  (Addgene inc)


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    Structured Review

    Addgene inc plasmid vectors plenticas9 t2a gfp
    Plasmid Vectors Plenticas9 T2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+sgrna+gfp/tet-pLKO-sgRNA-puro+(Plasmid+%23104321)/pmc12782865-48-1-4
    Average 94 stars, based on 16 article reviews
    plasmid vectors plenticas9 t2a gfp - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis
    Article Snippet: LINC00632 TSS-targeting sgRNA were designed in Benchling to target within 100 bases proximal to the annotated TSS for ENST00000498732 or ENST00000602535. .. All sgRNA sequences were cloned into pLKO-sgRNA-GFP (gift of Dr. Brian Brown, Mount Sinai School of Medicine), pLentiguide-Puro (Addgene # 52963), or Lenti-sgRNA(MS2)-Puro (Addgene #73795) by golden gate cloning of in vitro hybridized and phosphorylated oligos into BbsI or BsmBI cloning sites. sgRNA sequences are available in Supplementary Information : Oligos. pLentiguide-Puro and pLKO-sgRNA-GFP constructs were used in conjunction with pLentiCas9-Blast. .. Lenti-sgRNA(MS2)-Puro constructs were used in conjuction with Lenti-MS2-P65-HSF1-Hygro and Lenti-dCas9-VP64-Blast. shRNA shRNA sequences were cloned into Tet-pLKO-Puro (Addgene # 21915) by standard cloning methods.

    Cloning:

    Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis
    Article Snippet: LINC00632 TSS-targeting sgRNA were designed in Benchling to target within 100 bases proximal to the annotated TSS for ENST00000498732 or ENST00000602535. .. All sgRNA sequences were cloned into pLKO-sgRNA-GFP (gift of Dr. Brian Brown, Mount Sinai School of Medicine), pLentiguide-Puro (Addgene # 52963), or Lenti-sgRNA(MS2)-Puro (Addgene #73795) by golden gate cloning of in vitro hybridized and phosphorylated oligos into BbsI or BsmBI cloning sites. sgRNA sequences are available in Supplementary Information : Oligos. pLentiguide-Puro and pLKO-sgRNA-GFP constructs were used in conjunction with pLentiCas9-Blast. .. Lenti-sgRNA(MS2)-Puro constructs were used in conjuction with Lenti-MS2-P65-HSF1-Hygro and Lenti-dCas9-VP64-Blast. shRNA shRNA sequences were cloned into Tet-pLKO-Puro (Addgene # 21915) by standard cloning methods.

    In Vitro:

    Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis
    Article Snippet: LINC00632 TSS-targeting sgRNA were designed in Benchling to target within 100 bases proximal to the annotated TSS for ENST00000498732 or ENST00000602535. .. All sgRNA sequences were cloned into pLKO-sgRNA-GFP (gift of Dr. Brian Brown, Mount Sinai School of Medicine), pLentiguide-Puro (Addgene # 52963), or Lenti-sgRNA(MS2)-Puro (Addgene #73795) by golden gate cloning of in vitro hybridized and phosphorylated oligos into BbsI or BsmBI cloning sites. sgRNA sequences are available in Supplementary Information : Oligos. pLentiguide-Puro and pLKO-sgRNA-GFP constructs were used in conjunction with pLentiCas9-Blast. .. Lenti-sgRNA(MS2)-Puro constructs were used in conjuction with Lenti-MS2-P65-HSF1-Hygro and Lenti-dCas9-VP64-Blast. shRNA shRNA sequences were cloned into Tet-pLKO-Puro (Addgene # 21915) by standard cloning methods.

    Construct:

    Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis
    Article Snippet: LINC00632 TSS-targeting sgRNA were designed in Benchling to target within 100 bases proximal to the annotated TSS for ENST00000498732 or ENST00000602535. .. All sgRNA sequences were cloned into pLKO-sgRNA-GFP (gift of Dr. Brian Brown, Mount Sinai School of Medicine), pLentiguide-Puro (Addgene # 52963), or Lenti-sgRNA(MS2)-Puro (Addgene #73795) by golden gate cloning of in vitro hybridized and phosphorylated oligos into BbsI or BsmBI cloning sites. sgRNA sequences are available in Supplementary Information : Oligos. pLentiguide-Puro and pLKO-sgRNA-GFP constructs were used in conjunction with pLentiCas9-Blast. .. Lenti-sgRNA(MS2)-Puro constructs were used in conjuction with Lenti-MS2-P65-HSF1-Hygro and Lenti-dCas9-VP64-Blast. shRNA shRNA sequences were cloned into Tet-pLKO-Puro (Addgene # 21915) by standard cloning methods.

    other:

    Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
    Article Snippet: Lenti-sgRNA(MS2)-Puro constructs were used in conjuction with Lenti-MS2-P65-HSF1-Hygro and LentidCas9-VP64-Blast. shRNA shRNA sequences were cloned into Tet-pLKO-Puro (Addgene # 21915) by standard cloning methods.



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